TITLE Michaelis-Menten Enzyme-Substrate Reaction
: Simulation Resources, Inc.
: Reference - Segel, Enzyme Kinetics, Wiley, 1975
:   Chapter 2.
: 11/29/99

PARAMETER
{
  kf1 = 10
  kb1 = 10
  kfp = 1
  kbp = 1e-6
  Etotal = 1
}

INDEPENDENT
{
  time FROM 0 TO 10 WITH 100
}

ASSIGNED
{
  net_rate
}

STATE
{
  E     FROM 0 TO 10 START 1
  S     FROM 0 TO 10 START 10
  ES    FROM 0 TO 10 START 0
  P     FROM 0 TO 10 START 0
}

PLOT E, S, P, ES VS time

KINETIC ESreaction
{
~ E + S <-> ES          (kf1, kb1 )
~    ES <-> E + P       (kfp, kbp )
  net_rate = f_flux - b_flux
CONSERVE E + ES = Etotal
}

BREAKPOINT
{
  SOLVE ESreaction
}